Saturday, January 29, 2011

EMG

Electromyography (EMG) is an experimental technique concerned with the development, recording and analysis of myoelectric signals. Myoelectric signals are formed by physiological variations in the state of muscle fiber membranes

Typical benefits of EMG are:
  • · EMG allows to directly “look” into the muscle
  • · It allows measurement of muscular performance
  • · Helps in decision making both before/after surgery
  • · Documents treatment and training regimes
  • · Helps patients to “find” and train their muscles
  • · Allows analysis to improve sports activities
  • · Detects muscle response in ergonomic studies
FACTORS INFLUENCING THE EMG SIGNAL:

1) Tissue characteristics

The human body is a good electrical conductor, but unfortunately the electrical conductivity varies with tissue type, thickness, physiological changes and temperature. These conditions can greatly vary from subject to subject (and even within subject) and prohibit a direct quantitative comparison of EMG amplitude parameters calculated on the unprocessed EMG signal.

2) Physiological cross talk

Neighboring muscles may produce a significant amount of EMG that is detected by
the local electrode site. Typically this “Cross Talk” does not exceed 10%-15% of the
overall signal contents or isn’t available at all. However, care must been taken for
narrow arrangements within muscle groups. ECG spikes can interfere with the EMG
recording, especially when performed on the upper trunk / shoulder muscles. They are
easy to see and new algorithms are developed to eliminate them.

3) Changes in the geometry between muscle belly and electrode site

Any change of distance between signal origin and detection site will alter the EMG
reading. It is an inherent problem of all dynamic movement studies and can also be
caused by external pressure.

4) External noise

Special care must be taken in very noisy electrical environments. The most
demanding is the direct interference of power hum, typically produced by incorrect
grounding of other external devices.

5) Electrode and amplifiers

The selection/quality of electrodes and internal amplifier noise may add signal
contents to the EMG baseline. Internal amplifier noise should not exceed 5 Vrms.
Most of these factors can be minimized or controlled by accurate preparation and
checking the given room/laboratory conditions.

PROCEDURE OVERVIEW:

During the test, one or more small needles (also called electrodes) are inserted
through the skin into the muscle.

· Needle electrodes to study electrical activity of motor units
· Surface electrodes to study the electrical activity of muscles

EMG measures the electrical activity of muscle during rest, slight contraction, and
forceful contraction. Muscle tissue does not normally produce electrical signals
during rest. When an electrode is inserted, a brief period of activity can be seen on the
oscilloscope, but after that, no signal should be present.

After all of the electrodes have been inserted, you may be asked to contract the
muscle, for example, by lifting or bending your leg. The action potential (size and
shape of the wave) that this creates on the oscilloscope provides information about the
ability of the muscle to respond when the nerves are stimulated. As the muscle is
contracted more forcefully, more and more muscle fibers are activated, producing
action potentials.

ANALYSIS:

A healthy muscle will show no electrical activity (no signs of action potential) during
rest, only when it contracts. However, if the muscle is damaged or has lost input from
nerves, it may have electrical activity during rest. When it contracts its electrical
activity may produce abnormal patterns.

An abnormal EMG result may be a sign of a variety of muscle or nerve disorders,
including polymyositis (an inflammatory muscle disease that causes decreased muscle
power), muscular dystrophy (a chronic genetic disease that progressively affects
muscle function), myasthenia gravis (a genetic or immune disorder that occurs at the
point where the nerve connects with the muscle), and myotonic (stiff) muscles.

DETERMINATION OF CONDUCTION VELOCITIES IN MOTOR NERVES

THEORY:

Nerve conduction velocity (NCV) test is a measurement of the speed of conduction of
an electrical impulse through a nerve. NCV can determine nerve damage and
destruction.

During the test, the nerve is stimulated, usually with surface electrode patches
attached to the skin. Two electrodes are placed on the skin over the nerve. One
electrode stimulates the nerve with a very mild electrical impulse with pulse duration
of 0.2 to 0.5 m/s and the other electrode records it. The resulting electrical activity is
recorded by another electrode. This is repeated for each nerve being tested.

The nerve conduction velocity (speed) is then calculated by measuring the distance
between electrodes and the time it takes for electrical impulses to travel between
electrodes. This elapsed time is called latency.

The measurement of conduction velocity in motor nerves is used to indicate the
location and type of nerve lesion.

PROCEDURE:

  1. The EMG electrode and the stimulating electrode are placed at two points on the skin, separated by a known distance (L1).
  2. A brief electrical pulse is applied through the stimulating electrode.
  3. The action potential picked up by the EMG electrode is displayed on the software screen along with the stimulating impulse.
  4. The latency, between the stimulating impulse and muscle’s action potential is measured. (T1)
  5. Now, the two electrodes are repositioned with the distance of separation as (L2) such that L2 <>
  6. The latency is now measured (T2)
  7. Record your findings on the data sheet
  8. Calculate the conduction velocity.
  9. Repeat the test for different nerves.
ANALYSIS:

The speed of nerve conduction is related to the diameter of the nerve and the degree
of myelination (a myelin sheath is a type of "insulation" around the nerve). A
normally functioning nerve will transmit a stronger and faster signal than a damaged
nerve.

In general, the range of normal conduction velocity will be approximately 50 to 60
meters per second. However, the normal conduction velocity may vary from one
individual to another and from one nerve to another.

Abnormal results may be caused by some sort of neuropathy (damage to the nerve)
that can result from a contusion or traumatic injury to a nerve. Various diseases can
also cause the impulses to slow down.

Nerve conduction velocity is often used along with an EMG to differentiate a nerve
disorder from a muscle disorder. NCV detects a problem with the nerve whereas an
EMG detects whether the muscle is functioning properly in response to the nerve's
stimulus.

Diseases or conditions that may be evaluated with NCV include, but are not limited
to, the following:

· Guillain-BarrĂ© syndrome - a condition in which the body's immune system
attacks part of the peripheral nervous system. The first symptoms may include
weakness or tingling sensations in the legs.

· carpal tunnel syndrome - a condition in which the median nerve, which runs
from the forearm into the hand, becomes pressed or squeezed at the wrist by
enlarged tendons or ligaments. This results in pain and numbness in the
fingers.

· Charcot-Marie-Tooth disease - a hereditary neurological condition that affects
both the motor and sensory nerves. One characteristic is weakness of the foot
and lower leg muscles.

· herniated disc disease

· chronic inflammatory polyneuropathy and neuropathy - conditions resulting
from diabetes or alcoholism

· sciatic nerve problems

· pinched nerves

· peripheral nerve injury

Nerve conduction studies may also be performed to identify the cause of symptoms
such as numbness, tingling, and continuous pain.

Heart Sounds

Auscultation of the heart means to listen to and study the various sounds arising from the heart as it pumps blood. These sounds are the result of vibrations produced when the heart valves close and blood rebounds against the ventricular walls or blood vessels. The heart sounds may be heard by placing the ear against the chest or by using a stethoscope. The vibrations producing the sounds can be visually displayed through the use of a heart sound microphone and physiological recorder to produce a phonocardiogram. There are four major heart sounds, but only the first two can be heard without use of special amplification.

  • First heart sound. Produced at the beginning of systole when the atrioventricular (AV) valves close and the semilunar (SL; the aortic and pulmonary) valves open. This sound has a low-pitched tone commonly termed the lub sound of the heartbeat.
  • Second heart sound. Occurs during the end of systole and is produced by the closure of the SL valves, the opening of the AV valves, and the resulting vibrations in the arteries and ventricles. Owing to the higher blood pressures in the arteries, the sound produced is higher pitched than the first heart sound. It is commonly referred to as the dub sound.
  • Third heart sound. Occurs during the rapid filling of the ventricles after the AV valves open and is probably produced by vibrations of the ventricular walls.
  • Fourth heart sound. Occurs at the time of atrial contraction and is probably due to the accelerated rush of blood into the ventricles.
RELATIONSHIP BETWEEN HEART SOUNDS AND ECG:

  • FIRST HEART SOUND Coincide with R wave of ECG
  • SECOND HEART SOUND Coincide with the ending part of T wave of ECG
HEART VALVE FAILURE DISEASES

Aortic Stenosis:

Here the blood is ejected from the left ventricle through a small opening of the aortic
valve. Because of the resistance to ejection, the pressure in the left ventricle rises.
This causes turbulent blood flow. This turbulent blood impinging the aortic valve
causes intense vibration; it produces loud murmur (sounds related to non laminar flow
of blood in the heart).

Aortic Regurgitation:

No sound is heard during systole, but during diastole blood flows backward from the
aorta into the left ventricles, causing a blowing murmur. This is produced due to the
valves are damaged.

Mitral Regurgitation:
Here blood flows backward through the Mitral valve during systole. This produces
sound during systole.

Mitral Stenosis:

Here the blood passes with difficulty from the left atrium into the left ventricle due to
pressure difference. It produces murmur which is very weak.

ECG

BACKGROUND:

  • Sum of the electrical signals from the cardiac muscle as recorded on the surface of the body.
  • Pattern of the electrical activity depends on the orientation of the electrodes and the electrical activity of the cardiac cells.
EINTHOVEN TRIANGLE:

Willem Einthoven (1860-1927) attempted to explain the principles of the ECG in
scientific terms. In Einthoven's triangle, the heart may be considered to lie at the
centre of an equilateral triangle and the corners of the triangles are the effective
sensing points - the right arm, left arm and left leg electrodes.

CONVENTIONAL ECG ELECTRODE DERIVATIONS:

· 12 standard leads

· ECG is recorded:
  • Bipolar recording: between two points of the body (= bipolar recording)
  • Unipolar recording: between one point of the body (different electrode) and ground (indifferent electrode)
  • 6 limb leads: I, II, III (bipolar) and aVR, aVL, aVF (unipolar)
  • 6 precordial leads: V1 - V6 (unipolar)
LEADS CONNECTION:

Standard Limb Leads: I, II, III; bipolar, form a set of axes 60° apart
  • Lead I: Composed of negative electrode on the right arm and positive electrode on the left arm.
  • Lead II: Composed of negative electrode on the right arm and positive electrode on the left leg.
  • Lead III: Composed of negative electrode on the left arm and positive electrode on the left leg.
Augmented Voltage Leads:

aVR, aVL aVF; unipolar ; form a set of axes 60° apart but are rotated 30° from the axes of the standard limb leads.

· aVR: Exploring electrode located at the right shoulder.
· aVL: Exploring electrode located at the left shoulder.
· aVF: Exploring electrode located at the left foot.

Reference Point for Augmented Leads: The opposing standard limb lead; i.e., that
standard limb lead whose axis is perpendicular to the particular augmented lead.

Chest Leads: Vl, V2, V3, V4, V5, V6, explore the electrical activity of the heart in
the horizontal plane; i.e., as if looking down on a cross section of the body at the level
of the heart. These are exploring leads.

ECG curve:

  • P wave: Atrial depolarization (Small, rounded and upright)
  • QRS complex: Ventricular depolarization (Spiked with one or more deflections from the baseline)
  • T wave: Ventricular repolarization (Broad, rounded. if .QRS. then must be a .T. wave)
  • PR segment: AV nodal delay
  • ST segment: Ventricles are contracting and emptying the action potential of ventricular muscle cells in plateau phase
  • TP interval: Ventricle cells at rest, ventricular filling.
NORMAL VALUES FOR AMPLITUDES AND DURATIONS OF
IMPORTANT ECG PARAMETERS:

Amplitude:

P – wave 0.25 mV
R – wave 1.60 mV
Q – wave 25% of R wave
T – wave 0.1 to 0.5 mV

Duration:

P – R interval - 0.12 to 0.22 s
Q – T interval - 0.35 to 0.44 s
S – T interval - 0.05 to 0.15 s
P – wave interval - 0.11 s
QRS interval - 0.09 to 0.10 s

Links:

http://library.med.utah.edu/kw/ecg/
http://www.anaesthetist.com/icu/organs/heart/ecg/Findex.htm
http://nobelprize.org/educational/medicine/ecg/

Thursday, January 6, 2011

Blood Gas analysis (part 2)

Po2 electrode

History

Leland Clark (Professor of Chemistry, Antioch College, Yellow Springs, Ohio, and Fels Research Institute, Yellow Springs, Ohio) had developed the first bubble oxygenator for use in cardiac surgery. However, when he came to publish his results, his article was refused by the editor since the oxygen tension in the blood coming out from the device could not be measured. This instigated Clark to develop the oxygen electrode

Principle

  • Based on Redox reaction
  • Cathode (Pt) is reduced
  • Anode (Ag/Agcl) is oxidised
  • The resulting current linearly proportional to oxygen concentration
  • Operating voltage 0.68V (Since in V I characteristics of Po2 electrode around 0.6 to 0.7V current is constant)
Construction

Membrane - Polystyrene

Complete Blood Gas Analyzer

Separate amplifier for each electrode output
Sample size : 25uL
Response time: 1 - 5 mins

Accurate measurement of following parameters
a) pH
b) pCO2
c) pO2
d) Haematocrit and Hemoglobin
e) Electrolytes Sodium,Potassium andChloride Ca++ and Magnesium
f) Lactate
g)The equipment should possess electrodes with long life at least 2 years

Electrodes placed in temperature controlled chamber

Students activity

Design a temp control circuit to be utilized for Blood gas analyzer. The aim is to reduce / increase temperature if it changes from set-point.

Blood Gas analysis (part 1)

Blood gas analysis, also called arterial blood gas (ABG) analysis, is a procedure to measure the partial pressure of oxygen (O2) and carbon dioxide (CO2) gases and the pH (hydrogen ion concentration) in arterial blood. Oxygen content (O2CT), oxygen saturation (SaO2) and bicarbonate (RCO3 -) values are also measured.

Blood is most commonly drawn from the radial artery because it is easily accessible, can be compressed to control bleeding, and has less risk for occlusion. The femoral artery (or less often, the brachial artery) is also used, especially during emergency situations or with children. Blood can also be taken from an arterial catheter already placed in one of these arteries.

The syringe is pre-packaged and contains a small amount of heparin, to prevent coagulation or needs to be heparinised, by drawing up a small amount of heparin and squirting it out again. Once the sample is obtained, care is taken to eliminate visible gas bubbles, as these bubbles can dissolve into the sample and cause inaccurate results.

The sealed syringe is taken to a blood gas analyzer. If the sample cannot be immediately analyzed, it is chilled in an ice bath in a glass syringe to slow metabolic processes which can cause inaccuracy. Samples drawn in plastic syringes are not iced and are analyzed within 30 minutes

Purpose
  • To evaluate gas exchange in the lungs.
  • To assess integrity of the ventilatory control system.
  • To determine the acid-base level of the blood.
  • To monitor respiratory therapy
Normal ABG values fall within the following ranges:
  • PaO2: 75 to 100 mm Hg
  • PacO2: 35 to 45 mm Hg
  • pH: 7.35 to 7.45
  • O2CT: 15% to 22%
  • SaO2: 95% to 100%
  • HCO3 -: 24 to 28 mEq/L.
pH electrode

Conventional Glass electrode
Sensitivity 59mV/pH
Use of Syringe electrodes

pCo2 electrode

Principle:

  • Conventional pH electrode covered with rubber membrane
  • water is kept in between membrane and electrode
  • Diffused Co2 mixes with water to form H2CO3
  • H2CO3 dissociates into H+ and HcO3- ions
  • This H+ ions are sensed by pH electrode

pH = log HCO3 - log k - log a - log PCO2

In commercial electrode rubber is replaced by Teflon membrane and water replaced by sodium bicarbonate solution.


References:

  • Medical Instrumentation and design, John Webster, Page No: 442
  • http://en.wikipedia.org/wiki/Arterial_blood_gas

Generalized Medical Instrumentation System

The major difference between this system and a conventional instrumentation system is:

The source of the signals (measurand) is a living tissue or energy is applied to living tissue.

Measurand

Physical quantity, property, or condition that is being measured by the
system.

* most important issue : accessibility
- internal (blood pressure), on body surface (ECG, EEG)
- emanate from the body (infra-red radiation)
- derived from a tissue sample (blood or biopsy)

Medically important measurands

• Biopotentials (ECG, EEG, EMG, EOG, etc.)
• Pressure, flow, dimensions (imaging)
• Displacement (velocity dx/dt, acceleration d2x/d2t, and force =
md2x/d2t)
• Impedance, temperature and chemical concentration

The measurand may be localized to a specific organ or anatomical

Sensor

* The transducer or sensor should only respond to the form of energy present in the measurand to the exclusion of all others!
* The sensor should interface with the living system to minimize the energy extracted and being minimally invasive!

Signal conditioning

Usually the sensor output can not directly drive the display, therefore
signal processing or conditioning is required

Examples of signal processing:
1. Impedance matching
2. Amplification
3. Filtering
4. Mathematical mapping
5. Linearizing
6. Analog-to-digital conversion (ADC)
7. Digital-to-analog conversion (DAC)
8. Signal averaging to reduce noise (i.e. evoked response)
9. Transformation (time domain
frequency domain)
10. Compensation for undesirable sensor characteristics
11. Etc.

Output displays

Examples of output displays:
1. Numerical
2. Graphical
3. Discrete
4. Continuous
5. Permanent or temporary

• Most displays rely on our vision, but auditory sense is also sometimes used (for example, Doppler ultrasonic signals)

• User controls and output displays should conform to human factors engineering guidelines for the design of medical devices

Auxiliary Elements

*Calibration signal with the properties of the measurand should be applied to the sensor input or as early in the signal processing chain as possible

**Many forms of feedback (automatic or manual) may be required to elicit the measurand, to adjust the sensor and signal conditioner and to direct the flow of output (display, storage, transmission)

***Data storage for signal conditioning or examination of alarm conditions or implementation of different processing algorithms

**** Data communication transmission of patient data to remote display at nurse’s station and medical center

Operation Modes

1. Direct and Indirect Modes
• Direct: Measurand directly to sensor
- readily accessible or
- acceptable invasive procedure
For example: direct blood pressure measurement

• Indirect: measurand not accessible
- Use another measurand with known relation to the desired one
- Use some form of energy or material that interacts with the
desired measurand to generate a new accessible one

For example:
Cardiac output (volume of blood pumped/min by the heart)
- Measurements of respiration & blood gas concentration
- Dye dilution
- Morphology of internal organs determined from X-rays

2. Sampling or Continuous Modes
Sampling: Parameters that change slowly do not require continuous measurements

For example: body temperature, ionic concentrations, etc.

• Continuous: Parameters that change fast enough to require continuous measurements

For example: ECG, EEG, EMG, respiratory gas flow, etc.
Note: Frequency content of the measurand, the objective of the measurement, the condition of the patient and the potential liability of the physician influence how often data should be acquired

3. Generating and Modulating Sensors

Generating: Produce output from energy taken directly from measurand

For example: photovoltaic cell (output voltage related to irradiation)

• Modulating: Measurand changes flow of energy from an external source that affects the output of a sensor

For example: photoconductive cell (apply external power to the sensor to measure changes in resistance with irradiation)

4. Analog and Digital Modes

Analog: Continuous (parameter takes on any value within the dynamic range)

For example: Parameters that change fast enough to require continuous measurements: ECG, EEG, EMG, respiratory gas flow, etc.

• Digital: Discrete (parameter takes on a finite number of different values)

* Most sensors are analog (i.e., strain gages, thermistors, etc.)
* Very few sensors are digital in nature (i.e., shaft encoders)

5. Real-time and delayed-time Modes

Real-time: Sensors must acquire signals as they actually occur

• Output is not always displayed immediately, because some types of signal processing (i.e. averaging, transformations, etc) require considerable amount of date before production of final results

Delayed-time Often acceptable (short delays) unless urgent feedback & control depend on output

• Cell cultures provide an example where several days of delay may be required before an output is obtained!


VIVA QUESTIONS FOR DESIGN PROJECT LAB (CYCLE I)

1. What is a counter?

2. What are the types of counter?

3. Difference between BCD and Binary counter?

4. Difference between positive and negative edge triggering?

5. What is the use of batch counter?

6. What is the maximum count of a BCD counter?

7. What is a decoder?

8. Construct a BCD to seven segment decoder (using logical gates)?

9. What is the need for BCD to seven segment decoder?

10. What are the types of displays?

11. Compare common anode and cathode LED?

12. Mention the steps in designing a counter?

13. Identify the internal components of IC7490 and IC7447?

14. What are TTL IC’s?

15. What is ADC?

16. What is DAC?

17. Mention the types of ADC?

18. Mention the types of DAC?

19. What is the resolution of DAC?

20. What is the advantage of using R-2R over weighted resistor method?

21. Define conversion time, settling time, monotonocity.

22. Which is the fastest ADC? Why?

23. Give some examples of monolithic ADC and DAC?

24. Write the output expression for a N bit DAC?

25. What is sampling?

26. Define Sampling theorem.

27. What is quantization?

28. What is aliasing?

29. What is the significance of 3½ digits?

30. Explain briefly the operation of dual slope DVM?

31. Mention the value of integrating current?

32. How will you choose the integrating resistor and capacitor?

33. What is the operating frequency?

34. What are the ways of connecting oscillator to ICL 7107?

35. What is the use of test pin in ICL 7107?

36. How will you choose auto zero capacitor?

37. What is auto zero phase?

38. Mention the guide lines for choosing reference voltage in ICL 7107?

39. How will you generate a negative supply from +5V for ICL 7107?

40. What is the calibrator?

41. Mention the components of ICL 7107?

42. Identify the operating voltage, temperature condition for ICL 7107?

43. What is meant by regulation?

44. Define load and line regulation.

45. Mention the IC fixed positive voltage regulation?

46. Mention the IC fixed negative voltage regulation?

47. What is the need for variable regulators?

48. What is the need for step down transformer?

49. Compare iron and air core transformers.

50. Define Transformer Utilization Factor.

51. Define turns ratio.

52. What are the types of rectifier?

53. What is meant by ripple factor?

54. How will you choose a diode based on current rating?

55. What is the need for filter?

56. What are the types of filter?

57. What is the maximum input voltage range for IC 78xx and IC 79xx?

58. What is meant by dropout voltage?

59. What is telemetry?

60. What are the types of telemetry?

61. Mention the standard ranges for current, voltage and pressure telemetry.

62. Mention the transducer available for measurement of temperature?

63. Which temperature transducer has highest sensitivity?

64. Which temperature transducer has highest stability?

65. What is maximum possible temperature range of PT100?

66. Write the calendar equation?

67. Write the equation of resistance for RTD and Thermister?

68. Mention the need and advantage of Instrumentational amplifier?

69. What are the types of V/I converter?

70. What is the gain of instrumentational amplifier?

71. How will you test IC741 for proper working?

72. Mention monolithic instumentaional amplifier?

73. What is servo mechanisms?

74. What is linear regulator?

75. What is switching regulator?

76. What is the difference between buck and boost converter?

77. What are the factors to be considered when designing a regulated voltage supply?

78. Define PIV

79. What is the use of zero comparator in 3½ digit voltmeter?

80. Derive the output voltage for the following digital inputs

a) 1000

b) 0100

c) 0010

d) 0001 in R-2R ladder network.